AI Article Synopsis
- Arsenic exposure affects millions worldwide through contaminated water and the environment, necessitating a biological response for detoxification.
- Researchers aimed to understand the regulation of transcription at the arsenic response locus in budding yeast, using a technique called CRISPR-ChAP-MS to analyze proteins involved in this process.
- The study identified 40 key proteins, highlighting the roles of the SAGA histone acetyltransferase and the SWI/SNF chromatin remodeling complex in organizing chromatin structure necessary for activating gene transcription and aiding in arsenic detoxification.
Article Abstract
Arsenic exposure is a global health problem. Millions of people encounter arsenic through contaminated drinking water, consumption, and inhalation. The arsenic response locus in budding yeast is responsible for the detoxification of arsenic and its removal from the cell. This locus constitutes a conserved pathway ranging from prokaryotes to higher eukaryotes. The goal of this study was to identify how transcription from the arsenic response locus is regulated in an arsenic dependent manner. An affinity enrichment strategy called CRISPR-Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) was used, which provides for the proteomic characterization of a targeted locus. CRISPR-ChAP-MS was applied to the promoter regions of the activated arsenic response locus and uncovered 40 nuclear-annotated proteins showing enrichment. Functional assays identified the histone acetyltransferase SAGA and the chromatin remodelling complex SWI/SNF to be required for activation of the locus. Furthermore, SAGA and SWI/SNF were both found to specifically organize the chromatin structure at the arsenic response locus for activation of gene transcription. This study provides the first proteomic characterization of an arsenic response locus and key insight into the mechanisms of transcriptional activation that are necessary for detoxification of arsenic from the cell.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557609 | PMC |
| http://dx.doi.org/10.1080/15592294.2019.1580110 | DOI Listing |
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