Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification.

The harvest of a bench scale high cell density fermentation of Pichia pastoris, using standard laboratory equipment, to process products from supernatant is described. The process consists of a centrifugation step and a depth filtration, followed by a (sterile) membrane filtration. The procedure yields supernatant ready for the next purification step such as chromatography.