AI Article Synopsis
- The study focused on improving the process of doubling chromosomes in wheat through anther culture using colchicine.
- Optimal concentrations of colchicine were found to be 100 and 1000 mg/l, with significant improvements in doubling indices and reduced embryo structures.
- The best results for chromosome doubling occurred when colchicine was applied 20 days after inoculation, with a notable increase in success when used for 3 days.
Article Abstract
The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductions in embryo-like structures as well as higher doubling indices were found. Similar results were obtained with the high- and low-responding genotypes. Colchicine (100 mg/l), added 5 and 20 days after inoculation for 1 and 3 days increased the induction response, but this value was reduced when colchicine was added 10 or 15 days after inoculation. The doubling effect was similar to the control, except for a significant increase with the 3-day application 20 days after inoculation. The highest success index was reached when colchicine was added to the culture medium after 20 days.
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| http://dx.doi.org/10.1007/s002990050520 | DOI Listing |
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