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Customised 96-ocular TaqMan (iCAM) microarray PCR card for rapid diagnosis of microbial keratitis.
BMJ Open Ophthalmol
December 2024
Department of Ophthalmology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK
Aim: To validate the diagnostic performance of a custom 96-micro-organism TaqMan PCR card (iCAM) for microbial keratitis (MK) from a single corneal epithelial sample.
Methods: Patients over the age of 18 referred to Cambridge University Hospital with MK were recruited in this single-site prospective cohort study between September 2021 and January 2023. An ocular-specific, customised microarray card (iCAM) was constructed according to primer and probe nucleotide sequences developed in our department to detect bacteria, viruses, and fungi commonly implicated in MK using a single corneal epithelial sample.
Molecular detection and genetic analysis of species isolated from bovine clinical mastitis in India.
Vet Res Forum
September 2024
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
Bovine clinical mastitis is an economically important disease in dairy industry worldwide resulting in reduction of milk yield and quality. Among mycotic mastitis, spp. are commonly occurring opportunistic mycosis in immunocompromised animals.
View Article and Find Full Text PDFStudy of the Antimicrobial Potential of the , , cf. , nov., , and against Gram-Positive, Gram-Negative Bacteria, and Mycobacteria.
Mar Drugs
September 2023
Research Institute of Clinical and Experimental Lymphology-Filial of the Institute of Cytology and Genetics, Timakova 2, Novosibirsk 630060, Russia.
Article Synopsis
- * This study isolated various gram-positive and gram-negative bacteria from tuberculosis patients and evaluated the antibacterial potential of extracts from specific cyanobacteria and microalgae.
- * Findings indicate that while these extracts can inhibit bacterial growth, they can also promote it under certain conditions; however, fucoxanthin, a compound from these organisms, showed significant antibacterial properties in both in vitro and mouse models.
Efficacy of Quaternary Ammonium Compounds for Control of Individual and Mixed Cultures of with High- and Low-Quaternary Ammonium Compounds Resistance.
Foodborne Pathog Dis
July 2023
Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada.
is a well-characterized micro-organism in scientific literature. Similarly, quaternary ammonium compounds (QACs) are historical sanitizers in food processing. However, the use of QACs has been questioned due to bacterial resistance in some studies.
View Article and Find Full Text PDFβ-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for infected bone defect treatment.
PLoS One
December 2022
Department of Orthopedics, Hospital of Zhejiang People's Armed Police (PAP), Hangzhou, Zhejiang, China.
The repair of infected bone defects remains a clinical challenge. Staphylococcus aureus is a common pathogenic micro-organism associated with such infections. Gentamycin (GM) is a broad spectrum antibiotic that can kill S.
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