AI Article Synopsis
- The calvarial injection method is used to test substances on bone by injecting them under the skin of a mouse's skull.
- This method enables researchers to assess both bone resorption and formation in a straightforward living model.
- Histological analysis, often using glycolmethacrylate-embedded tissue, is conducted to study the effects, including details on various histochemical and staining techniques.
Article Abstract
This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
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| http://dx.doi.org/10.1007/978-1-4939-8997-3_21 | DOI Listing |
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