Genome Engineering Approaches to Improve Nosokomycin A Production by B38.3.

Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.