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Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system. | LitMetric

AI Article Synopsis

  • Genome editing technology, particularly using the CRISPR-Cas9 system, allows for precise genetic modifications in various species, including the common marmoset, a valuable primate model in research.
  • The research team established an effective knock-in (KI) method for marmoset embryonic stem cells (ESCs), significantly improving KI efficiency through homologous recombination.
  • The findings indicate that marmoset ESCs have a naturally high efficiency for repairing DNA double-strand breaks, paving the way for advanced genetic modifications and functional gene studies in this species.

Article Abstract

Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRISPR-Cas9 system. The use of CRISPR-Cas9, mediated by homologous recombination (HR), enhanced the KI efficiency in marmoset ESCs. Furthermore, we succeeded in performing KI in early-stage marmoset embryos. In the course of the experiments, we found that HR in the marmoset ESCs is innately highly efficient. This suggested that the marmoset possesses a repair mechanism for DNA double-strand breaks. The current study will facilitate the generation of genetically modified marmosets and gene function analysis in the marmoset.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365532PMC
http://dx.doi.org/10.1038/s41598-018-37990-wDOI Listing

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