AI Article Synopsis

  • The text discusses an opportunistic bacterial pathogen known for its rising antimicrobial resistance and its risk of causing infections in neonatal intensive care units (NICUs), necessitating quick infection control measures.
  • Researchers developed a whole-genome multilocus sequence typing (wgMLST) method using 299 whole-genome sequences, creating a comprehensive system that includes 9,377 gene loci, facilitating detailed tracking of the pathogen's spread.
  • Validation of this wgMLST method with 111 clinical isolates demonstrated strong alignment with epidemiological data, indicating its potential for effective monitoring and containment of outbreaks related to this pathogen.

Article Abstract

is an opportunistic bacterial pathogen. It is notorious for its increasing antimicrobial resistance and its potential to cause outbreaks of colonization and infections, predominantly in neonatal intensive care units (NICUs). There, its spread requires rapid infection control response. To understand its spread, detailed molecular typing is key. We present a whole-genome multilocus sequence typing (wgMLST) method for Using a set of 299 publicly available whole-genome sequences (WGS), we developed an initial wgMLST system consisting of 9,377 gene loci. This included 1,455 loci occurring in all reference genomes and 7,922 accessory loci. This closed system was validated using three geographically diverse collections of consisting of 111 clinical isolates implicated in nosocomial dissemination events in three hospitals. The validation procedure showed a full match between epidemiological data and the wgMLST analyses. We set the cutoff value for epidemiological (non)relatedness at 20 different alleles, though for the majority of outbreak-clustered isolates, this difference was limited to 4 alleles. This shows that the wgMLST system for provides prospects for successful future monitoring for the epidemiological containment of this opportunistic pathogen.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440794PMC
http://dx.doi.org/10.1128/JCM.01652-18DOI Listing

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