Metabolite profiling in complex biological matrices such as serum requires high-throughput technologies capable of accurate and reproducible quantitative analysis and detection of slight differences in metabolite concentrations. Gas chromatography-mass spectrometry (GC-MS) is widely used for characterizing the metabolome. This chapter summarizes the necessary preparatory steps required to profile the metabolome using GC-MS. While this chapter focuses on evaluating polar metabolites in serum samples, the methods can be adapted to quantify nonpolar metabolites in other biological matrices.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-9027-6_13 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Uncovering Antiviral Potential of Cistus ladanifer Extracts Against HSV-1 and SARS-CoV-2 by In Vitro and In Silico Analysis.
Chem Biodivers
January 2025
National Center for Energy Sciences and Nuclear Techniques: Centre National de l'Energie des Sciences et des Techniques Nucleaires, Biology and Medical Research Unit, Rabat 10001, Morocco, 10001, Rabat, MOROCCO.
Infectious diseases remain a major global health concern. Cistus ladanifer, a plant commonly employed in Moroccan traditional medicine, has been identified as a potential antiviral candidate. This study aimed to evaluate the antiviral activity of C.
View Article and Find Full Text PDFA Gas Chromatography-Mass Spectrometry Method to Determine Tramadol Abuse Using Urine Samples.
Cureus
December 2024
Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bangalore, IND.
Background The synthetic opioid tramadol is widely used as a pain reliever. Unlike other opioids, it is used freely worldwide, unaffected by international controls resulting in abuse and accidental intoxication. Analytical methods are necessary to prove tramadol abuse because 30% of the drug is excreted unchanged.
View Article and Find Full Text PDFAntioxidant, Antimicrobial, Anticancer, and Molecular Docking Insights into Pancratium maritimum Seeds and Flowers: A Phytochemical Approach.
ChemistryOpen
January 2025
Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, Brazil.
This study investigates the antioxidant, antimicrobial, and anticancer properties of Pancratium maritimum L. in Sp. Pl.
View Article and Find Full Text PDFEarly and Mid-Term Disposition of α-PVP and its unknown Metabolites in Urine and Oral Fluid Through a Multi-Analytical Hyphenated Approach Following a Single Non-Controlled Administration to Healthy Volunteers.
AAPS J
January 2025
National Center On Addiction and Doping, National Institute of Health, Viale Regina Elena 299, 00161, Rome, Italy.
Nowadays, synthetic cathinones (SCs) is the second more representative subclass of New Psychoactive Substances, accounting for 104 analogues in the illegal market. Since its first report in 2011, α-pyrrolidinovalerophenone (α-PVP) gained popularity among drug users, provoking an increased number of intoxications. Nonetheless, pharmacokinetics data is still limited in the literature.
View Article and Find Full Text PDFChemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta.
Sci Rep
January 2025
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box.2455, Riyadh, 11451, Saudi Arabia.
Tuta absoluta is one of the most destructive pests of tomatoes. Chemical insecticides used to control this leafminer harm all organisms, increasing the risk to public health and the environment. Developing natural alternatives, such as bioinsecticides formulated from essential plant oils, is a key strategy to address this problem.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!