AI Article Synopsis
- Researchers created a simple and cost-effective method for freezing zebrafish sperm using dry ice for 20 minutes and a basic solution composed of glucose, KCl, and Tris.
- The sperm freezing method showed an average fertilization rate efficiency between 10% and 20%.
- Testing was done at two different sites in different countries, resulting in consistent outcomes that demonstrate the method's reliability and practicality.
Article Abstract
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200 mM glucose, 40 mM KCl, 30 mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method.
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| http://dx.doi.org/10.1089/zeb.2018.1696 | DOI Listing |
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