AI Article Synopsis
- Rift Valley fever virus (RVFV) is a mosquito-borne disease causing significant harm, like abortion in livestock, leading to economic losses in Africa and concerns over its spread and potential bioterrorism.
- Recent changes in vector distribution and international trade have made RVFV a transboundary animal disease of high concern, necessitating improved detection methods.
- The study introduces a new chromogenic pan-RVFV in situ hybridization (ISH) assay that uses FFPE tissues to reliably detect RVFV strains while maintaining important tissue morphology for visual analysis.
Article Abstract
Sporadic outbreaks of Rift Valley fever virus (RVFV), a zoonotic, mosquito-borne , cause abortion storms and death in sheep and cattle resulting in catastrophic economic impacts in endemic regions of Africa. More recently, with changes in competent vector distribution, growing international trade, and its potential use for bioterrorism, RVFV has become a transboundary animal disease of significant concern. New and sensitive techniques that determine RVFV presence, while lessening the potential for environmental contamination and human risk, through the use of inactivated, noninfectious samples such as formalin-fixed, paraffin-embedded (FFPE) tissues are needed. FFPE tissue hybridization (ISH) enables the detection of nucleic acid sequences within the visual context of cellular and tissue morphology. Here, we present a chromogenic pan-RVFV ISH assay based on RNAscope technology, which is able to detect multiple RVFV strains in FFPE tissues, enabling visual correlation of RVFV RNA presence with histopathologic lesions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/vbz.2018.2383 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pasteurization temperatures effectively inactivate Rift Valley fever viruses in milk.
J Virol
January 2025
ASTRE, CIRAD, INRAE, University of Montpellier, Montpellier, France.
Exploiting venom toxins in paratransgenesis to prevent mosquito-borne disease.
Parasit Vectors
January 2025
Centre for Snakebite Research and Interventions, Liverpool School of Tropical Medicine, Liverpool, UK.
Mosquitoes are responsible for the transmission of numerous pathogens, including Plasmodium parasites, arboviruses and filarial worms. They pose a significant risk to public health with over 200 million cases of malaria per annum and approximately 4 billion people at risk of arthropod-borne viruses (arboviruses). Mosquito populations are geographically expanding into temperate regions and their distribution is predicted to continue increasing.
View Article and Find Full Text PDF"Without a man's decision, nothing works": Building resilience to Rift Valley fever in pastoralist communities in Isiolo Kenya.
PLoS One
January 2025
Institute of Anthropology, Gender and African Studies, University of Nairobi, Nairobi, Kenya.
Rift Valley Fever (RVF) is a zoonotic disease that affects both livestock and humans. Men and women in pastoralist communities are vulnerable to RVF risk exposure because of their different roles and reliance on livestock products. This study sought to understand how ownership and decision-making in pastoralist male and female-headed households influence coping mechanisms and resilience to Rift Valley fever (RVF), using the three resilience capacities of absorptive, adaptive, and transformative.
View Article and Find Full Text PDFA Novel HTNV Budding Inhibitor Interferes the Interaction Between Viral Glycoprotein and Host ESCRT Accessory Protein ALIX.
J Med Virol
February 2025
Department of Microbiology, School of Basic Medicine, Air Force Military Medical University, Xi'an, China.
Virus budding is a critical step in the replication cycle of enveloped viruses, closely linked to viral spread, disease progression, and clinical outcomes. The budding of many enveloped RNA viruses is facilitated by the hijacking of the host endosomal sorting complex required for transport (ESCRT) proteins through viral late domains. These late domains are essential for progeny virus production and are highly conserved, making the interaction between late domains and host ESCRT proteins a potential target for the development of antiviral therapeutics.
View Article and Find Full Text PDFIntroduction: Recent Rift Valley fever (RVF) epidemiology in eastern Africa region is characterized by widening geographic range and increasing frequency of small disease clusters. Here we conducted studies in southwestern (SW) Uganda region that has since 2016 reported increasing RVF activities.
Methods: A 22-month long hospital-based study in three districts of SW Uganda targeting patients with acute febrile illness (AFI) or unexplained bleeding was followed by a cross-sectional population-based human-animal survey.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!