tuber (GET) is a popular traditional Chinese medicines (TCMs). In this study, response surface methodology (RSM) with a Box⁻Behnken design (BBD) was performed to optimize the extraction parameters of gastrodin-type components (gastrodin, gastrodigenin, parishin A, parishin B, parishin C and parishin E). Different from the conventional studies that merely focused on the contents of phytochemical, we gave consideration to both quantitative analysis of the above six components by HPLC and representative bioactivities of GET, including antioxidation and protection of human umbilical vein endothelial cells (HUVEC). Four independent variables (ethanol concentration, liquid-material ratio, soaking time and extraction time) were investigated with the integrated evaluation index of phytochemical contents. With the validation experiments, the optimal extraction parameters were as follows: ethanol concentration of 41%, liquid⁻solid ratio of 28.58 mL/g, soaking time of 23.91 h and extraction time of 46.60 min. Under the optimum conditions, the actual standardized comprehensive score was 1.8134 ± 0.0110, which was in accordance with the predicted score of 1.8100. This firstly established method was proved to be feasible and reliable to optimize the extraction parameters of the bioactive components from GET. Furthermore, it provides some reference for the quality control and extraction optimization of TCMs.
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http://dx.doi.org/10.3390/molecules24030547 | DOI Listing |
Biomech Model Mechanobiol
January 2025
Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA.
When infants are admitted to the hospital with skull fractures, providers must distinguish between cases of accidental and abusive head trauma. Limited information about the incident is available in such cases, and witness statements are not always reliable. In this study, we introduce a novel, data-driven approach to predict fall parameters that lead to skull fractures in infants in order to aid in determinations of abusive head trauma.
View Article and Find Full Text PDFBMC Oral Health
January 2025
Oral Medicine, Periodontology, Diagnosis and Oral Radiology Department, Faculty of Dentistry, Mansoura University, Mansoura City, 33516, Egypt.
Objective: This systematic review and meta-analysis aim to evaluate the therapeutic potential of boric acid as a local drug delivery agent in the treatment of periodontitis.
Methods: Following PRISMA guidelines, we registered a comprehensive protocol with PROSPERO. By employing PICOS criteria, we evaluated randomized controlled trials assessing the effects of subgingival boric acid application alongside non-surgical periodontal therapy in treatment of periodontitis.
BMC Ecol Evol
January 2025
National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bellary Road, Bengaluru, 560065, India.
Background: Trait variation is shaped by functional roles of traits and the strength and direction of selection acting on the traits. We hypothesized that in butterflies, sexually selected colouration is more variable owing to condition-dependent nature and directional selection on sexual ornaments, whereas naturally selected colouration may be less variable because of stabilising selection. We measured reflectance spectra, and extracted colour parameters, to compare the amount of variation in sexually versus naturally selected colour patches across wing surfaces and sexes of 20 butterfly species across 4 families (Nymphalidae, Papilionidae, Pieridae, Lycaenidae).
View Article and Find Full Text PDFSci Rep
January 2025
Botany and Microbiology Department, Faculty of Science, Damietta University, New Damietta, 34517, Egypt.
Ganoderma mushrooms have a variety of pharmacological activities and may have antitumor effects. Therefore, the antitumor activity of the methanolic fruiting body extracts of three Ganoderma spp. will be evaluated by estimating cell viability, cell cycle parameters and the mode of cellular death.
View Article and Find Full Text PDFJ Dairy Sci
January 2025
Department of Population Medicine, University of Guelph, Guelph, ON, Canada N1G 2W1. Electronic address:
Bovine respiratory disease (BRD) is one of the most common diseases observed in dairy calves in both the pre- and post-weaning periods. Despite its common occurrence, there has been no formal synthesis (e.g.
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