Summary: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.

Availability And Implementation: nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial.

Supplementary Information: Supplementary data are available at Bioinformatics online.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748755PMC
http://dx.doi.org/10.1093/bioinformatics/btz084DOI Listing

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