Hepatoprotective potential of extract for carbon tetrachloride induced hepatotoxicity and oxidative damage in rats.

We investigated the hepatoprotective potential of extract for CCI induced liver damage. We used six groups of rats: group 1, untreated control; group 2, CCl treated (hepatotoxic); group 3, treated with 150 mg/kg ; group 4, treated with 300 mg/kg ; group 5, treated with CCl + 150 mg/kg ; and group 6, treated with CCl + 300 mg/kg . Liver damage was produced by injection of 1 ml/kg CCI twice/week. Extracts of , 150 and 300 mg/kg/day, were administered for 8 weeks. The effects of were assessed by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transferase (GGT) and total bilirubin (T-BIL) levels, and the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the liver. The histology and immunohistochemistry of liver tissue were evaluated using hematoxylin and eosin staining, and caspase 3 and 8-OHdG immunostaining. extract produced significant reductions in elevated levels of ALT, AST, GGT and T-BIL and increased levels of GPx and SOD in rats treated with CCl. extract decreased CCl induced 8-OHdG formation and caspase 3 activation significantly in hepatocytes, especially at the 150 mg/kg dose. Our findings demonstrate the potential efficacy of for attenuating CCl induced hepatotoxicity and oxidative damage.