Calreticulin (CRT) is an multifunctional resident endoplasmic reticulum (ER) luminal protein implicated in regulating a variety of cellular processes, including Ca storage/mobilization and protein folding. These multiple functions may be carried out by different CRT genes and protein isoforms. The plant CRT family consist of three genes: CRT1 and CRT2 classified in the common subclass (CRT1/2), and CRT3. These genes are highly conserved during evolution and encode three different protein products (CRT1, 2 and 3). The aim of the current study was to conduct a comparative analysis and sequence-based classification of the plant CRT genes. We used nucleotide and amino acid sequences to phylogenetically cluster the genes and examine potential glycosylation patterns. Additionally, we analyzed phylogenetic relationships within the CRT subclasses. Finally, we analyzed intraspecific CRT duplication events among mono- and dicotyledon species. Our results confirm that each of the CRT genes exist in multiple copies in plant genomes, and that CRT gene duplication is a widespread process in plants.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ympev.2019.01.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of the CaCRT gene family and function of CaCRT1 under low-temperature stress in pepper (Capsicum annuum L.).
Sci Rep
January 2025
College of Horticulture and Forestry, Tarim University, Alar, 843300, China.
To explore CRT gene family members and their responses to low-temperature stress, bioinformatics methods were used to identify the CRT gene family in pepper. In this study, a total of 4 CRT gene family members were identified by screening. The genes were found to be located on different chromosomes, and phylogenetic tree and collinearity analyses were performed.
View Article and Find Full Text PDFNetwork pharmacology, molecular docking, and experimental verification to reveal the mechanism of colquhounia root tablet in the treatment of diabetic nephropathy.
Biochem Biophys Res Commun
December 2024
Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. Electronic address:
Article Synopsis
- Diabetic nephropathy (DN) is a common complication of diabetes, and Colquhounia Root Tablet (CRT) has shown potential therapeutic effects, although its mechanisms are not fully understood.
- Using various databases, researchers identified 163 therapeutic targets of CRT related to DN and found key targets like STAT3 and IL6 through protein-protein interaction analysis.
- The study highlighted the AGEs/RAGE signaling pathway as significant for CRT's effectiveness against DN and confirmed its protective effects on kidney cells via experimental treatments, although further studies are required to assess the safety and efficacy in real-life scenarios.
Molecular responses of paddy field carp (Cyprinus carpio) in the agricultural heritage to major environmental factors in paddy fields.
Comp Biochem Physiol Part D Genomics Proteomics
December 2024
Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, PR China; Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, PR China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, PR China. Electronic address:
Article Synopsis
- The Qingtian paddy field carp has been domesticated for over 1200 years and has adapted to shallow-water conditions in paddy fields.
- Research involved transcriptome sequencing of the carp's hepatopancreas at different temperatures, revealing 3154 differentially expressed genes linked to various metabolic pathways.
- The study highlights the role of protein processing in the endoplasmic reticulum and the HIF-1 signaling pathway in helping PF-carp maintain cell stability under high temperature and hypoxic conditions.
FBLN1 regulates ferroptosis in acute respiratory distress syndrome by reducing free ferrous iron by inhibiting the TGF-β/Smad pathway.
PLoS One
December 2024
Department of Pulmonary and Critical Care Medicine, Shanghai Pudong New Area People's Hospital, Shanghai, China.
Background: Acute respiratory distress syndrome (ARDS) / acute lung injury (ALI) is a serious medical disease characterized by pulmonary dysfunction and inflammation. This study aims to determine the main molecular modules linked to ARDS and investigate the role of Fibulin-1 (FBLN1) in regulating ferroptosis in ARDS.
Methods: Weighted Gene Co-expression Network Analysis (WGCNA) was employed on the GSE263867 dataset to find key modules associated with ALI.
Article Synopsis
- The obligate intracellular parasite replicates within a compartment called the parasitophorous vacuole (PV) and utilizes a protein ingestion pathway to take in nutrients from the host cell's cytosol, initiated by the protein GRA14.
- A genome-wide CRISPR screen revealed that mutants lacking components of this ingestion pathway (GRA14, CPL, or CRT) are forced to rely more on alternative metabolic pathways to survive, such as pyrimidine and fatty acid biosynthesis.
- Analysis showed that these ingestion-deficient mutants had lower levels of key nutrients and growth defects when amino acids were scarce, indicating that the ingestion pathway plays a crucial role in nutrient acquisition during resource-limited conditions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!