AI Article Synopsis
- Mutations in isocitrate dehydrogenase (IDH) I and II in gliomas lead to the buildup of 2-hydroxyglutarate (2-HG) in tumor cells, potentially impacting prognosis and surgical outcomes.* -
- The study introduces a quick method using nanoelectrospray ionization (nESI) to measure 2-HG levels, facilitating real-time determination of IDH mutation status during surgery.* -
- By combining tandem mass spectrometry with low-resolution mass spectrometry, the technique provides a reliable way to analyze mutation status in minutes, enhancing surgical decision-making.*
Article Abstract
Isocitrate dehydrogenase (IDH) I and II mutations in gliomas cause an abnormal accumulation of 2-hydroxyglutarate (2-HG) in these tumor cells. These mutations have potential prognostic value in that knowledge of the mutation status can lead to improved surgical resection. Information on mutation status obtained by immunohistochemistry or genomic analysis is not available during surgery. We report a rapid extraction nanoelectrospray ionization (nESI) method of determining 2-HG. This should allow the determination of IDH mutation status to be performed intraoperatively, within minutes, using a miniature mass spectrometer. This study demonstrates that the combination of tandem mass spectrometry with low-resolution mass spectrometry allows this analysis to be performed with confidence. Graphical Abstract.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450702 | PMC |
| http://dx.doi.org/10.1007/s00216-019-01632-5 | DOI Listing |
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