AI Article Synopsis
- Affinity purification combined with mass spectrometry is a preferred method for identifying protein interactions in natural cellular environments.
- This report presents a detailed protocol for tandem affinity purification (TAP) that utilizes FLAG and HA peptide tags.
- The focus is on improving yield and specificity, along with guidance on how to automate the TAP process.
Article Abstract
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure. © 2019 by John Wiley & Sons, Inc.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579647 | PMC |
| http://dx.doi.org/10.1002/cpps.84 | DOI Listing |
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