Previous diet affects the amount but not the type of bait consumed by an invasive ant.

Background: Recent research on multiple invasive ant species has revealed the importance of carbohydrates for achieving high activity levels and outcompeting native ants. However, comparatively little is known about the role of diet and macronutrient preferences for uptake of insecticidal baits used to control invasive ants. We tested whether diet affected yellow crazy ant (Anoplolepis gracilipes Fr Smith) survival and behavior, and whether bait preference would be complementary to past diet.

Results: We found that colonies fed only crickets for 28 days had fewer live workers and queens, and less brood per live queen than colonies fed crickets + honeydew but did not differ significantly from colonies fed only honeydew. Colonies that had been fed only crickets were more active (as assessed by interaction with a novel object), retrieved 16-17 times more bait per worker overall, and consumed more of the six bait types than cricket + honeydew and honeydew-only fed colonies. However, prior diet did not affect bait choice. The two highest sugar bait formulations combined accounted for most of the bait consumed across all treatments (cricket-only 74.8% ± 28.1; cricket + honeydew 69.2% ± 12.4; honeydew-only 62.5% ± 30.4).

Conclusion: Yellow crazy ant colonies fare better without protein than without carbohydrates. Yellow crazy ants ate the most bait when fed only crickets but did not choose baits complementary to their previous diet. Baits in a sugar-rich carrier may be most effective for the control of yellow crazy ants, regardless of the relative availability of macronutrients. © 2019 Society of Chemical Industry.