Evaluation of differential qPE9-1/DEP1 protein domains in rice grain length and weight variation.

Background: qPE9-1/DEP1, encoding a G protein γ subunit, has multiple effects on plant architecture, grain size, and yield in rice. The qPE9-1 protein contains an N-terminal G gamma-like (GGL) domain, a putative transmembrane domain, and a C-terminal cysteine-rich domain. However, the roles of each domain remain unclear.

Results: In the present study, we focused on the genetic effects of different domains of qPE9-1 in the regulation of grain length and weight. We generated a series of transgenic plants expressing different truncated qPE9-1 proteins through constitutive expression and clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 strategies. Phenotypic analysis indicated that the complete or long-tailed qPE9-1 contributed to the elongation of grains, while the GGL domain alone and short-tailed qPE9-1 led to short grains. The long C-terminus of qPE9-1 including two or three C-terminal von Willebrand factor type C domains effectively repressed the negative effects of the GGL domain on grain length and weight. qPE9-1-overexpressing lines in a Wuxianggeng 9 (carrying a qpe9-1 allele) background showed increased grain yield per plant, but lodging occurred in some years.

Conclusions: Manipulation of the C-terminal length of qPE9-1 through genetic engineering can be used to generate varieties with various grain lengths and weights according to different requirements in rice breeding. The genetic effects of qPE9-1/qpe9-1 are multidimensional, and breeders should take into account other factors including genetic backgrounds and planting conditions in the use of qPE9-1/qpe9-1.