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Analysis of Methylation Status of Plant MicroRNAs. | LitMetric

Analysis of Methylation Status of Plant MicroRNAs.

Methods Mol Biol

State Key Laboratory of Genetic Engineering and Institute of Plant Biology, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, China.

Published: June 2019

Small noncoding RNAs of 20-30 nucleotides in length are key mediators of gene silencing. 2'-O-Methylation on the 3' terminal nucleotide of several types of small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs) in plants, PIWI-interacting RNAs (piRNAs) in animals, and some siRNAs in Drosophila and Caenorhabditis elegans, provides a key protective mechanism against 3' tailing- and trimming-mediated destabilization. The methylation reaction is catalyzed by the small RNA methyltransferase HUA ENHANCER 1 (HEN1). In this chapter, we describe a detailed protocol for analyzing 3' end methylation status of plant miRNAs, which can be applicable to other types of small RNAs as well.

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Source
http://dx.doi.org/10.1007/978-1-4939-9042-9_21DOI Listing

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