Morphological, biological, and genetic characteristics of a virulent phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It was found to infect some strains giving clear plaques. The genome of this phage is composed of 50,910 bp and contains 89 ORFs. Importantly, none of the predicted ORFs shows any similarity with known pathogenic factors that would prevent its use in medicine. Genome sequence analysis of vB-EcoS-95 revealed 74% similarity to genomic sequence of phage pSf-1. Compared to pSf-1, phage vb-EcoS-95 does not infect strains and has an efficient bacteriolytic activity against some strains. One-step growth analysis revealed that this phage has a very short latent period (4 min), and average burst size of 115 plaque forming units per cell, which points to its high infectivity of host cells and strong lytic activity. The bacteriolytic effect of vB-EcoS-95 was tested also on biofilm-producing strains. These results indicate that vB-EcoS-95 is a newly discovered phage that may be potentially used to control the formation of biofilms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340994 | PMC |
| http://dx.doi.org/10.3389/fmicb.2018.03326 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anthropogenic Drivers of Small-Island Effects in Urban Remnant Woody Plants.
Plants (Basel)
December 2024
College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao 266109, China.
The positive relationship between species richness and area is a fundamental principle in ecology. However, this pattern deviates on small islands, where species richness either changes independently of area or increases at a slower rate-a phenomenon known as the Small-Island Effect (SIE). While the SIE has been well documented in natural ecosystem, its presence in highly fragmented and disturbed urban ecosystem remains unexplored, posing challenges for urban vegetation conservation.
View Article and Find Full Text PDFClinical Cases of Tick-Borne Diseases in Dogs During the Autumn-Winter Season in Poland.
Pathogens
December 2024
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Tick-borne diseases (TBDs) pose a growing threat to companion animals, especially dogs, due to the increasing abundance of tick populations in Europe, driven by climate change, urbanization, and the mobility of humans and animals. This study aimed to assess the prevalence of tick-borne pathogens in clinically ill dogs suspected of having developed TBDs during the autumn-winter season, as well as to detect pathogens in ticks collected during the same period in the Warmian-Masurian Voivodeship in Poland. A total of 30 dogs with clinical symptoms of babesiosis and 45 ticks from dogs were acquired for this study.
View Article and Find Full Text PDFFirst Detection of West Nile Virus Lineage 2 in Vectors in Croatia.
Pathogens
December 2024
Department of Biology, University Josip Juraj Strossmayer of Osijek, 31000 Osijek, Croatia.
The West Nile virus (WNV) has recently become more widespread, posing a threat to both human and animal health. In Western Europe, most outbreaks have been caused by WNV lineage 1, while in Eastern Europe, WNV lineage 2 has led to human and bird mortality. The ability to appropriately manage this threat is dependent on integrated surveillance and early detection.
View Article and Find Full Text PDFTracing the Origin, Spread, and Molecular Evolution of Dengue Type 1 Cases That Occurred in Northern Italy in 2023.
Pathogens
December 2024
Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
The dengue virus (DENV) is a mosquito-borne flavivirus endemic to many tropical and subtropical regions. Over the past few decades, the global incidence of dengue has risen dramatically, with the virus now present in over 100 countries, putting nearly half of the world's population at risk. This increase is attributed to several factors, including urbanization, climate change, and global travel, which facilitate the spread of both the virus and its mosquito vectors.
View Article and Find Full Text PDFThe Brain in Context: A Scoping Review and Concept Definition of Neuro-Informed Policy and Practice.
Brain Sci
December 2024
Queensland Brain Institute, The University of Queensland, St. Lucia, Brisbane 4072, Australia.
Background/objectives: Among the developmental sciences, discovery in neuroscience has underpinned research innovations and made a significant contribution to knowledge translation. With the growth of neuroscience discovery, policymakers and practitioner workforces have adopted 'neuro-informed' in decisions targeting the delivery of human, social, and economic wellbeing.
Methods: In this scoping review, we examined the use and conceptualization of neuro-informed policy and practice (NPP) over the last two decades.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!