To study the action of methylation on transformation efficiency, pA2 was methylated in vitro with M. Sau3A, M. AluI, M. MspI, M. HpaII, M. PvuII, M. BcnI, M. MvaI and transformed into Saccharomyces cerevisiae. The results suggest that efficiency of transformation depends on specificity of methylation, the nature of methylcytosine (m5C or m4C).
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