AI Article Synopsis
- Legionella pneumophila can enter a viable but non-culturable state to survive harsh conditions, making them hard to detect.
- Despite being non-culturable, these bacteria still show signs of life, like respiration and maintaining their cell wall.
- The paper presents a method for identifying these bacteria using Syto 9 and propidium iodide staining with flow cytometry.
Article Abstract
Legionella pneumophila, among other bacteria, may enter a viable but non-culturable state as a means for survival in stressful conditions. Bacterial cells in the viable but non-culturable state cannot grow on standard medium; however, they continue to exhibit characteristics that are associated with live cells, such as respiration, transcription, and cell wall integrity. The present paper outlines a detailed protocol for the detection of viable but non-culturable L. pneumophila cells via Syto 9 and propidium iodide staining coupled with flow cytometry.
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| http://dx.doi.org/10.1007/978-1-4939-9048-1_3 | DOI Listing |
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