The standard cultivation procedure for requires gas exchange and a microaerophilic atmosphere. A novel system using a commercially available cell culture device (Petaka G3; Celartia Ltd., Powell, OH) was assessed for long-term cultivation of a reference laboratory clone in normal air. Parasite growth during 30 days was similar, or better, in Petaka G3 than that in the standard cultivation method with gas exchange in a CO incubator. The successful cultivation of in the Petaka G3 device suggests that low O content available in hemoglobin and dissolved gas in the blood is sufficient for long-term cultivation. This finding may open the way to novel methods to cultivate and adapt field isolates to conditions with more ease.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447126 | PMC |
| http://dx.doi.org/10.4269/ajtmh.18-0527 | DOI Listing |
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