AI Article Synopsis
- - Malania oleifera, an endangered tree in China's Karst region, has seeds rich in valuable fatty acids, but genetic research is limited due to insufficient tools.
- - Researchers sequenced the tree's genome, generating over 186 Gb of DNA sequences, resulting in a high-quality genome assembly with 24,064 predicted protein-coding genes and significant repeat content.
- - This study presents the first complete genome and gene annotation for M. oleifera, paving the way for conservation efforts and deeper understanding of nervonic acid production.
Article Abstract
Background: Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree's seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools.
Findings: We generated 51 Gb and 135 Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10× Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51 Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10× Genomics reads. Identified repeats constituted ∼82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail.
Conclusions: Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377399 | PMC |
| http://dx.doi.org/10.1093/gigascience/giy164 | DOI Listing |
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