Hymenobacter persicinus sp. nov., a novel member of the family Hymenobacteraceae.

A bacterial strain, 1-3-3-3, was isolated from a soil sample collected in Jeollabuk-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be red-pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Hymenobacter in the family Hymenobacteraceae, with high levels of 16S rRNA sequence similarity with Hymenobacter algoricola VUG-A23a (98.0%), Hymenobacter knuensis 16F7C-2 (97.9%), Hymenobacter fastidiosus VUG-A124 (97.1%), Hymenobacter elongatus VUG-A112 (97.0%), Hymenobacter chitinivorans Txc1 (97.0%) and Hymenobacter aquaticus 16F3P (96.7%). Growth of strain 1-3-3-3 was observed at 10-30 °C, pH 6-8 and in the presence of 0-1.0% NaCl. The genomic G + C content was determined to be 61.6 mol %. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as iso-C (19.9%), summed feature 3 (Cω7c/Cω6c, 19.7%), summed feature 4 (iso-C I/anteiso-C B, 17.8%), Cω5c (12.5%) and anteiso-C (11.2%), and the major polar lipid was found to be phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the affiliation of strain 1-3-3-3 with the genus Hymenobacter. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 34%. The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 1-3-3-3 represents a novel species in the genus Hymenobacter, for which the name Hymenobacter persicinus sp. nov. is proposed. The type strain is 1-3-3-3 (= KCTC 52742 = JCM 32191).