AI Article Synopsis
Article Abstract
IL-1R-associated kinase 4 (IRAK4), a central TIR signaling mediator in innate immunity, can initiate a cascade of signaling events and lead to induction of inflammatory target gene expression eventually. In the present study, we cloned and characterized an IRAK4 orthologue from grass carp (Ctenopharyngodon idella). The full length cDNA of CiIRAK4 was 2057 bp with an ORF of 1422 bp encoding a polypeptide of 472 amino acids. Multiple alignments showed that IRAK4s were highly conserved among different species. Phylogenetic tree analysis revealed that CiIRAK4 shared high homologous with zebra fish IRAK4. Expression analysis indicated that CiIRAK4 was widely expressed in all tested tissues. It was significantly up-regulated after treatment with poly I:C, especially obvious in liver and spleen. Also, CiIRAK4 could be induced by poly I:C and LPS in CIK cells. Fluorescence microscopy assays showed that CiIRAK4 localized in the cytoplasm. RNAi-mediated knockdown and overexpression assays indicated that CiIRAK4 might have little effect on NF-kappa B p65 translocation from cytoplasm to nucleus, indicating that CiIRAK4 was dispensable for activation of NF-kappa B p65. In addition, IRAK4 promoted IRF5 nuclear translocation, which has nothing to do with the interaction between IRAK4 and IRF5. It suggested that fish IRAK4 kinase regulated IRF5 activity through indirect ways.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2019.01.031 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Relations between microplastic contamination and stress biomarkers under two seasonal conditions in wild carps, mullets and flounders.
Mar Environ Res
January 2025
ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia e Ecologia, (ECOTOX), Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal; CIIMAR / CIMAR-LA - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Research Team of Aquatic Ecotoxicology and One Health, and Research Team of Contaminant Pathways in Marine Environment, Terminal de Cruzeiros do Porto de Leixões, 4450-208, Matosinhos, Portugal. Electronic address:
Potential effects of microplastics (MP, plastic particles <5 mm) on the levels of multiple stress biomarkers were investigated in wild fish populations of Cyprinus carpio, Mugil cephalus, Platichthys flesus captured in the Minho River estuary located in the Iberian Peninsula. Specimens were collected in March and September 2018, corresponding to the end of winter and summer, respectively. Based on the concentration of MP determined by FT-IR analysis and morphological inspection, fishes from each species were divided into two groups: ≤0.
View Article and Find Full Text PDFTargeted and non-targeted metabolic analysis of chlorpromazine in grass carp as well as the in-silico and metabolomics toxicity assessment.
J Hazard Mater
January 2025
Jiangsu Institute for Food and Drug Control, 6 Beijing West Road, Nanjing 210008, China. Electronic address:
Chlorpromazine (CPZ) is an abused sedative that is extensively metabolized in organisms. However, the metabolic pathway of CPZ in aquatic organisms is still unclear. In this study, CPZ metabolites was analyzed in grass carp exposed to CPZ in the raising water using ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS).
View Article and Find Full Text PDFBiosafety and Withdrawal Period of In-Feed Administered Antiparasitic Drug Emamectin Benzoate in Cirrhinus mrigala (Hamilton, 1822).
J Vet Pharmacol Ther
January 2025
Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture (CIBA), Chennai, India.
Parasitic infestations are one of the most economically important disease conditions in the Indian major carps including mrigal, Cirrhinus mrigala. This study reported the biosafety and tissue withdrawal of in-feed administered antiparasitic drug, emamectin benzoate (EMB). To evaluate the biosafety of the drug, behaviour, growth and tissue changes in Cirrhinus mrigala was recorded the following in-feed administration of EMB up to 10 times (T1-50 μg kg fish day (1×), T2-125 μg kg fish day (2.
View Article and Find Full Text PDFAstragaloside IV can mitigate heat stress-induced tissue damage through modulation of the Keap1-Nrf2 signaling pathway in grass carp (Ctenopharyngodon idella).
Fish Shellfish Immunol
January 2025
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, Guangdong, 528225, China. Electronic address:
This study investigated the potential protective effect of AS-IV against heat stress-induced tissue damage in grass carp (Ctenopharyngodon idella). Grass carp were injected intraperitoneally with 0, 2, 4, and 8 mg/kg of AS-IV for three consecutive days, and then subjected to heat stress (35 ± 0.5 °C); thereafter, histopathological analyses of the liver and spleen were performed at 0, 6, 24, and 48 h, respectively.
View Article and Find Full Text PDFEpicatechin attenuates emamectin benzoate-induced liver injury in grass carp by activating Nrf2/GPX4 signaling pathway.
Fish Shellfish Immunol
January 2025
College of Animal Science and Technology, Jilin Agriculture Science and Technology University, Jilin City, China. Electronic address:
Emamectin benzoate (EMB) is an antibiotic insecticide pesticide modified from avermectin. In the current study, we performed an in-depth investigation of the protective effects of epicatechin on EMB-induced liver injury in common carps. The carps were cultured in an aquatic environment containing 2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!