Interaction between indium tin oxide nanoparticles and ferricytochrome c: Conformation, redox state, and adsorption scheme.

The conformations and redox states of ferricytochrome c, before and after adsorption onto the surface of the indium tin oxide (ITO) nanoparticles, are studied to reveal the interaction nature between the cytochrome c and the conducting metal oxide surface. The characterizations with resonance Raman scattering and UV-Vis absorption reveal that the change of pH at moderate ionic strength induces transitions of conformations and redox-states, which suggests that there is intramolecular electron transfer. The conformations of the cytochrome c species are maintained after adsorption onto or collision with the ITO surface, but the redox states change significantly, and the change depends on the surface structure of the ITO nanoparticle. The adsorption or collision processes are governed by the pH-dependent electrostatic interaction between the proteins and the buffer anions bound to the ITO surface. This adsorption scenario differs from the conventional ones.