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Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A β hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome.
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http://dx.doi.org/10.1126/science.aav7003 | DOI Listing |
J Surg Educ
March 2025
Afghan Clinic, San Francisco, California; Harvard Medical School, Boston, Massachusetts.
Objective: To develop an intervention addressing the unique needs of Afghan premedical students in successfully applying to and preparing for medical school in the U.S.
Design: A year-long virtual mentorship program was developed using the principles of community-based participatory research and cultural humility.
J Environ Manage
March 2025
Beijing Information Science and Technology University, School of Management Science and Engineering, Beijing, 102206, China; Beijing Knowledge Management Research Center, Beijing, 102206, China. Electronic address:
Achieving industrial symbiosis and zero emissions requires integrated frameworks for optimizing industrial waste valorization. However, existing studies often overlook the synergy between operational paradigms and multidimensional efficiency. This study bridges this gap through a mixed-methods investigation of 48 Chinese industrial waste resource (IWR) utilization centers, combining qualitative analysis of 1.
View Article and Find Full Text PDFJ Surg Res
March 2025
Section of Surgical Sciences, Vanderbilt University Medical Center, Nashville, Tennessee. Electronic address:
Introduction: Creating a "tension-free anastomosis" is a fundamental principle in safe bowel surgery. This review aims to summarize the current literature regarding the measurement and impact of tension on bowel anastomoses.
Methods: This scoping review was conducted using a systematic literature search in the PubMed, SCOPUS, and EMBASE databases.
Cardiooncology
March 2025
Department of Medicine and Population Health Research Institute, McMaster University, Hamilton, Canada.
Advances in the diagnosis and management of prostate cancer have significantly changed the disease landscape. While benefiting from better oncological outcomes, patients are now experiencing higher rates of non-cancer comorbidities, including cardiovascular disease. The increasing impact of cardiovascular disease in those with prostate cancer led to the expanding role of cardio-oncology professionals in enhancing the multidisciplinary care of these patients.
View Article and Find Full Text PDFBMC Pregnancy Childbirth
March 2025
Key Laboratory of Research and Development on Clinical Molecular Diagnosis for High-Incidence Diseases of Baise, Baise, 533000, China.
Objective: Gestational diabetes mellitus (GDM) seriously endangers the health of pregnant women and their offspring. Early prediction and diagnosis allow timely treatment of GDM, preventing adverse pregnancy outcomes and related diseases. This research aims to explore the predictive significance of miR-486-3p expression levels in diagnosing GDM in early pregnancy.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!