Synthesis of g-CN@CuMOFs nanocomposite with superior peroxidase mimetic activity for the fluorometric measurement of glucose.

Herein, a novel metal-organic framework (MOF) based nanocomposite with efficient catalytic behavior is reported including flake-like copper (II) MOF (CuMOF) and graphitic CN nanosheets (g-CN). The g-CN@MOF nanocomposite was simply prepared by solvothermal synthesis of CuMOF in the presence of g-CN. The characterization analyses using scanning electron microscopy (SEM), X-ray diffractometry (XRD) and some other techniques demonstrated a nano-porous flake-like structure for the synthesized CuMOF, which enveloped the g-CN nanosheets. Furthermore, the investigation of catalytic behavior of synthesized nanomaterial was implemented on HO based reactions. The fluorometric and colorimetric experimentations illustrated that the accompanying of g-CN with CuMOF had a remarkable positive effect on the catalytic behavior of obtained g-CN@MOF. This effect was described based on the improved affinity of nanocomposite to adsorb HO and also synergistic action of its components on the dissociation of HO to hydroxyl radicals. Finally, the analytical application of high catalytic activity of new g-CN@MOF was designed for the rapid and simple measurement of glucose in blood. After the enzymatic oxidation of glucose, the fluorometric method was applied for the analysis of produced HO using terephthalic acid as peroxidase substrate. The system led to the ultrasensitive glucose determination in the concentration range of 0.1-22 μM, with a detection limit (3S/m) of 59 nM.