AI Article Synopsis
- The study focused on understanding how and why certain bacteria have become more resistant to carbapenems, a type of antibiotic, by examining their genetic makeup.
- Researchers collected various clinical bacteria samples from patients at a military hospital in Sofia, Bulgaria, which displayed different levels of resistance to these antibiotics.
- The results indicated that the heightened resistance was linked to a greater number of copies of a specific gene (carbapenemase VIM-1), suggesting that more gene copies lead to stronger expression and resistance.
Article Abstract
The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among clinical isolates. The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing. A series of clinical isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of gene copies. We showed here that increased carbapenem resistance in may result from increased expression of the carbapenemase gene through multiplication of its copy number.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/mdr.2018.0365 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of carbapenem-resistant Klebsiella pneumoniae from blood cultures in Gaza Strip hospitals, Palestine.
Int J Med Microbiol
January 2025
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany. Electronic address:
Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a difficult to treat organism owing to limited therapeutic options. So far, little is known about the molecular characteristics of CRKP in Palestine.
Objectives: The aim of this study was to investigate the antimicrobial resistance patterns, multilocus sequence types (ST) and resistance genes among clinical K.
Prevalence of ESBL resistance genes and fecal carriage of multidrug-resistant Pseudomonas aeruginosa isolates from patients with chronic kidney disease at the Laquintinie Hospital in Douala, Littoral Region, Cameroon.
J Infect Public Health
January 2025
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address:
Background: Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium capable of causing severe infections in immunocompromised patients such as those suffering from chronic kidney disease (CKD). This study aimed to determine the resistance profile of Pseudomonas aeruginosa, and the prevalence of extended-spectrum β-lactamase (ESBL) resistance genes in patients with chronic kidney disease.
Methods: The prevalence of Pseudomonas aeruginosa was investigated in 458 patients, including 197 CKD patients and 261 patients suffering from gastrointestinal infections.
Detection methods for carbapenem-resistant Pseudomonas aeruginosa in surface water and wastewater.
Sci Total Environ
January 2025
National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands; Department of Biotechnology, Technical University Delft, Delft, the Netherlands. Electronic address:
Water systems can act as an important reservoir for Pseudomonas aeruginosa, which can pose public health risks during human contact. Carbapenem resistance is one of the most concerning resistances in P. aeruginosa making it a high-priority pathogen according to the World Health Organization (WHO), due to its ability to cause difficult-to-treat infections.
View Article and Find Full Text PDFEnhanced Killing of Methicillin-Resistant Staphylococcus aureus with Ceftaroline or Vancomycin in Combination with Carbapenems.
J Infect Dis
January 2025
Department of Pediatrics, University of California Irvine School of Medicine, Irvine, CA 92697, USA.
Background: Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia is associated with high rates of treatment failure, even when antibiotics showing in vitro susceptibility are used. Early optimization of therapy is crucial to reduce morbidity and mortality. Building on our previous research on carbapenem therapy for methicillin-susceptible S.
View Article and Find Full Text PDFBackground: Metallo-beta lactamase resistance is one of the carbapenem resistances that worsen the world nowadays. A new variant of carbapenem-resistant has only limited reports from Africa including Ethiopia. This study aimed to determine Metallo -ß- lactamase resistance Gram-negative bacteria in Hawassa University Comprehensive Specialized Hospital January-June 2023.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!