AI Article Synopsis
- The study explores using probiotic bacteria as a feed additive to slow down reproductive aging in hens, particularly focusing on their follicle development.
- Researchers investigated how Bacillus subtilis KATMIRA1933 affected the expression of vitellogenin genes and the number of follicles in 570-day-old hens, finding positive results.
- The study suggests that measuring vitellogenin mRNA levels can serve as a useful biomarker for assessing egg-laying ability in hens.
Article Abstract
A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen's egg-laying ability.
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| http://dx.doi.org/10.1007/s12602-019-9519-y | DOI Listing |
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