AI Article Synopsis
- SUGARWIN1 and SUGARWIN2 are sugarcane defense proteins that are activated in response to wounding and damage, leading to fungal cell death.
- The study reveals that these proteins significantly enhance plant defense against pathogens, with varying induction levels observed among different sugarcane varieties.
- Additionally, SUGARWIN1 shows multiple enzymatic activities (ribonuclease, chitosanase, and chitinase), while SUGARWIN2 is limited to chitosanase activity, suggesting that differences in their amino acid structures influence their functional specificity.
Article Abstract
SUGARWIN1 and 2 are defense proteins from sugarcane. Their gene expression is known to be induced in response to wound and damage. Although the recombinant SUGARWIN protein does not affect insect development, it promotes significant morphological and physiological changes in and , which lead to fungal cell death via apoptosis. In this study, we deepen our understanding of the role of SUGARWINs in plant defense and the molecular mechanisms by which these proteins affect fungi by elucidating their molecular targets. Our results show that SUGARWINs play an important role in plant defense against opportunistic pathogens. We demonstrated that are induced by , and the induction of SUGARWINs can vary among sugarcane varieties. The sugarcane variety exhibiting the highest level of induction exhibited a considerable reduction in infection. Furthermore, SUGARWIN1 exhibited ribonuclease, chitosanase, and chitinase activity, whereas SUGARWIN2 exhibited only chitosanase activity. This variable enzymatic specificity seems to be the result of divergent amino acid composition within the substrate-binding site.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330325 | PMC |
| http://dx.doi.org/10.3389/fpls.2018.01916 | DOI Listing |
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