Sieve-through vertical flow platform for efficient liquid exchange in particle-based assays.

Anal Chim Acta

NanoBio Lab, 31 Biopolis Way, The Nanos, Singapore, 138669, Singapore. Electronic address:

Published: March 2019

AI Article Synopsis

  • Particle-based assays are popular in biomedical fields but struggle with contamination from residual reagents during liquid exchanges.
  • A new sieve-through platform employs a porous membrane and absorbent pad to efficiently remove waste liquid, enhancing liquid exchange and reducing carry-over contamination.
  • The platform has been shown to improve particle-based ELISA assays for proteins and cells and allows high-throughput analysis through a sieve-array for concurrent sample testing.

Article Abstract

Particle-based assays are widely used in many biomedical applications. However, the performance of particle-based systems is often compromised by the carry-over contamination caused by the residual reagents during the liquid-exchange process. We have developed a sieve-through platform that utilizes a porous membrane to sieve out the particles, and an absorbent pad to remove the waste liquid by capillary force. The porous membrane is able to contain the liquid in the reaction chamber, and allows the waste liquid to flow through when it is brought into contact with the absorbent pad. The sieve-through platform is able to effectively remove the waste liquid, thereby achieving a more efficient liquid exchange as compared to the conventional process, and minimizing the carry-over contamination. In this study, we have determined the factors that affect the flow characteristics through the porous membrane on the sieve-through platform. We have shown that the sieve-through platform effectively reduces the carry-over contamination. In addition, we have shown particle-based ELISA on the sieve-through platform for the analysis of proteins and cells. We have further demonstrated the potential of the sieve-through platform for high-throughput analysis by presenting a sieve-array, which allows concurrent analysis of multiple samples in parallel. The sieve-through platform can significantly improve the performance of particle-based systems.

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Source
http://dx.doi.org/10.1016/j.aca.2018.11.020DOI Listing

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