Protein separation in the free-flow electrophoretic apparatus with 48 channels at both the inlet and outlet using "artificial" pH gradients was investigated. The separation was carried out in borate-mannitol pH gradients and in pH gradients created by the concentration gradient of boric acid in the solutions of borax and mannitol. The separation pattern depended on the ratio of the rate of sample injection to the flow-rate of solutions in the separation chamber and did not change when the protein concentration in the sample was changed. The protein separation in the pH gradient was better than in case of conventional free-flow electrophoresis. The free-flow electrophoretic apparatus with multi-channel inlet of the separation chamber is suitable for concentration of biological materials, e.g. proteins and bacterial cells.
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