Optogenetic approaches have gathered momentum in precisely modulating and interrogating cellular signalling and gene expression. The use of optogenetics on the outer cell surface to interrogate how cells receive stimuli from their environment, however, has so far not reached its full potential. Here we demonstrate the development of an optogenetically regulated membrane receptor-ligand pair exemplified by the optically responsive interaction of an integrin receptor with the extracellular matrix. The system is based on an integrin engineered with a phytochrome-interacting factor domain (OptoIntegrin) and a red light-switchable phytochrome B-functionalized matrix (OptoMatrix). This optogenetic receptor-ligand pair enables light-inducible and -reversible cell-matrix interaction, as well as the controlled activation of downstream mechanosensory signalling pathways. Pioneering the application of optogenetic switches in the extracellular environment of cells, this OptoMatrix-OptoIntegrin system may serve as a blueprint for rendering matrix-receptor interactions amendable to precise control with light.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325061 | PMC |
| http://dx.doi.org/10.1038/s42003-018-0264-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the Bone Marrow Lymphoid Microenvironment and Discovery of Prognostic Immune-Related Factors in Acute Myeloid Leukemia.
Int J Mol Sci
December 2024
Department of Hematology, Catholic Hematology Hospital, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
Given the limited comprehensive data on the bone marrow (BM) immune environment in acute myeloid leukemia (AML), we analyzed the distribution and phenotype of T cell subsets, including γδ T cells, and their immune checkpoint (IC) ligands on blasts. We performed multiparametric flow cytometry with BM samples taken from 89 AML patients at the time of diagnosis, remission, and relapse/refractory status after chemotherapy and 13 healthy controls (HCs) to identify immune-related risk factors. Compared to the HCs, the T cells of the AML patients exhibited exhausted features including higher TIGIT levels and similar levels of PD-1 and TIM-3.
View Article and Find Full Text PDFModulating Immune Responses: The Double-Edged Sword of Platelet CD40L.
Semin Thromb Hemost
October 2024
Department of Pharmacy, University of Bonn, Bonn, Germany.
Article Synopsis
- The CD40-CD40L interaction is crucial for regulating the immune system, affecting monocytes, T cells, and B cells, as well as antibody production.
- While traditionally linked to immune cells, activated platelets express most CD40L, playing a key role in communications with immune cells and helping to fight infections.
- However, CD40L can also contribute to harmful inflammation in conditions like HIV and sepsis, making it a double-edged sword; thus, targeting CD40L for treating inflammatory diseases requires careful consideration to avoid severe side effects.
GPR15-mediated T cell recruitment during acute viral myocarditis facilitated virus elimination and improved outcome.
Nat Cardiovasc Res
January 2024
Department of Cardiology and Angiology, University Heart Center Freiburg-Bad Krozingen, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Article Synopsis
- Viral myocarditis involves the immune response to virus invasion, but the role of the GPR15 receptor in heart disease remains unclear.
- Researchers found that mice lacking GPR15 had trouble eliminating coxsackievirus B3, which resulted in harmful heart changes and dysfunction.
- The study suggests that GPR15 is crucial for the timely recruitment of regulatory T cells, and its absence leads to a prolonged inflammatory response and worse cardiac outcomes.
Single-Cell RNA Sequencing Reveals a Unique Fibroblastic Subset and Immune Disorder in Lichen Sclerosus Urethral Stricture.
J Inflamm Res
August 2024
Department of Transfusion Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710038, People's Republic of China.
Purpose: Lichen sclerosus urethral stricture disease (LS USD) is a refractory and progressive disease primarily affecting the anterior urethra in males. Various potential etiological factors, such as genetics, autoimmunity, infection, and exposure to infectious urine, have been suggested. However, the accurate etiology of LS in the male urethra remains unclear.
View Article and Find Full Text PDFA Single Trophoblast Layer Acts as the Gatekeeper at the Endothelial-Hematopoietic Crossroad in the Placenta.
bioRxiv
July 2024
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.
During embryonic development the placental vasculature acts as a major hematopoietic niche, where endothelial to hematopoietic transition ensures emergence of hematopoietic stem cells (HSCs). However, the molecular mechanisms that regulate the placental hematoendothelial niche are poorly understood. Using a parietal trophoblast giant cell (TGC)-specific knockout mouse model and single-cell RNA-sequencing, we show that the paracrine factors secreted by the TGCs are critical in the development of this niche.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!