Aim: We describe the validation of an HPLC-MS/MS method to analyze ceftolozane and tazobactam simultaneously in saline matrixes.
Materials & Methods: An Agilent 1260 HPLC interfaced to an Agilent 6470 triple-quadrupole mass spectrometer was used for quantification. A reverse-phase column running a gradient of water and acetonitrile containing 0.1% formic acid mobile phase at a flow rate of 1.0 ml/min provided chromatographic fractionation. TazobactamN was used as the internal standard. The standard curves were linear over a range of 0.02-0.5 μg/ml.
Conclusion: This methodology represents a simple, reproducible approach to the determination of drug concentrations with accuracy and precision for pharmacokinetic studies undertaken with this recently US FDA-approved antimicrobial therapy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331755 | PMC |
| http://dx.doi.org/10.4155/fsoa-2018-0079 | DOI Listing |
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