Triterpenes from the Mushroom : Isolation, Structure Determination and Investigation in Bdelloid Rotifer Assays.

Twelve compounds (⁻) were isolated from the methanol extract of brick cap mushroom ( (Schaeff.) P. Kumm.). The structures of the compounds were elucidated using extensive spectroscopic analyses, including NMR and MS measurements. Lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol () and 8-hydroxy-13-oxo-9,11-octa-decadienoic acid () were identified as new natural products, together with ten known compounds, from which 3β-hydroxyergosta-7,22-diene (), demethylincisterol A2 (), cerevisterol (), 3β--glucopyranosyl-5,8-epidioxyergosta-6,22-diene (), fasciculol E (), and uridine () were identified in this species for the first time. The isolated triterpenes (, ⁻) were investigated for their toxicity in vivo using bdelloid rotifer assays. Most of the examined steroids in general showed low toxicity, although the effects of the compounds varied in a wider range from the non-toxic lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol () to the significantly toxic cerevisterol (), with substantial dependence in some cases on the presence of nutrient in the experimental environment.