Background: Due to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose.
Results: An effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill. Chlorides and nitrates of Al and Fe showed better synergistic effect with MA, especially AlCl, ascribing to the interaction between metal salt and oxygen-containing groups induced by MA. Comparative studies showed that MAMS pretreatment effectively changed the recalcitrant structural characteristics of lignocellulosic matrix and reduced the inhibitory action of lignin on enzymatic conversion of SCB. The increase in hydroxyl and carboxyl groups of lignin induced by MAMS pretreatment led to the increase of its hydrophilicity, which could weaken the binding force between cellulase and lignin and reduce the nonproductive binding of cellulase enzymes to lignin.
Conclusions: MAMS pretreatment significantly enhanced the enzymatic digestibility of polysaccharides substrate by overcoming biomass recalcitrance without the removal of lignin from enzymatic hydrolysis system.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327530 | PMC |
| http://dx.doi.org/10.1186/s13068-019-1354-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
INTERNATIONAL SYMPOSIUM ON RUMINANT PHYSIOLOGY: Rumen fungi, archaea and their interactions.
J Dairy Sci
January 2025
Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing, China 210095.
Anaerobic gut fungi (AGF) were the last phylum to be identified within the rumen microbiome and account for 7-9% of microbial biomass. They produce potent lignocellulases that degrade recalcitrant plant cell walls, and rhizoids that can penetrate the cuticle of plant cells, exposing internal components to other microbiota. Interspecies H transfer between AGF and rumen methanogenic archaea is an essential metabolic process in the rumen that occurs during the reduction of CO to CH by methanogens.
View Article and Find Full Text PDFAre cellulases slow? Kinetic and thermodynamic limitations for enzymatic breakdown of cellulose.
BBA Adv
December 2024
Novonesis, 2 Biologiens Vej, DK-2800 Lyngby Denmark.
Cellulases are of paramount interest for upcoming biorefineries that utilize residue from agriculture and forestry to produce sustainable fuels and chemicals. Specifically, cellulases are used for the conversion of recalcitrant plant biomass to fermentable sugars in a so-called saccharification process. The vast literature on enzymatic saccharification frequently refers to low catalytic rates of cellulases as a main bottleneck for industrial implementation, but such statements are rarely supported by kinetic or thermodynamic considerations.
View Article and Find Full Text PDFOcean acidification and global warming may favor blue carbon service in a Cymodocea nodosa community by modifying carbon metabolism and dissolved organic carbon fluxes.
Mar Pollut Bull
January 2025
Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI·MAR), Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.
Ocean acidification (OA) and global warming (GW) drive a variety of responses in seagrasses that may modify their carbon metabolism, including the dissolved organic carbon (DOC) fluxes and the organic carbon stocks in upper sediments. In a 45-day full-factorial mesocosm experiment simulating forecasted CO and temperature increase in a Cymodocea nodosa community, we found that net community production (NCP) was higher under OA conditions, particularly when combined with warming (i.e.
View Article and Find Full Text PDFCo-metabolism of Norfloxacin by Chlorella pyrenoidosa: Carbon source effects, biotransformation mechanisms, and key driving genes.
J Hazard Mater
December 2024
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
Co-metabolism with appropriate carbon sources has been demonstrated to effectively enhance the removal of ubiquitous recalcitrant micropollutant by microalgae. However, the specific impacts of carbon sources on the co-metabolism of antibiotics by microalgae remain insufficiently explored. In this study, transcriptomics, gene network analysis, extracellular polymeric substances (EPS), and enzymatic activity involved in co-metabolic pathways of norfloxacin (NFX), were systematically evaluated to investigate the underlying biological mechanisms involved in NFX co-metabolism by Chlorella pyrenoidosa.
View Article and Find Full Text PDFRecovery in soil carbon stocks but reduced carbon stabilization after near-natural restoration in degraded alpine meadows.
Sci Rep
December 2024
Grassland Technique Extension Station of Gansu Province, Lanzhou, 730000, Gansu, China.
Near-natural restoration is acknowledged as an effective strategy for enhancing soil organic carbon (SOC) sequestration in degraded grasslands. However, the alterations in SOC fractions, stability, and relative sequestration capacity after restoration of degraded alpine meadows remain uncertain. In this study, we utilized the degraded alpine meadows on the northeastern edge of the Tibetan Plateau as a research area, with grazing as the control (CK) and restoration of 20 years of banned grazing (BG) and growing season resting grazing (RG).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!