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Enhancement of silymarin and phenolic compound accumulation in tissue culture of Milk thistle using elicitor feeding and hairy root cultures. | LitMetric

AI Article Synopsis

  • The study tested the effects of chitosan, methyl jasmonate, and salicylic acid on the accumulation of silymarin and phenolic compounds in Milk thistle cultures, using various concentrations of these metabolite elicitors.
  • Increased concentrations of the elicitors significantly boosted the total silymarin content, particularly enhancing the accumulation of silybine A&B, which were absent in untreated callus cultures.
  • Hairy root cultures demonstrated superior production of phenolic compounds and antioxidant capacities compared to non-transformed and chemically treated cultures, suggesting they are effective for enhancing economically valuable metabolites.

Article Abstract

In the present study, the effects of the metabolite elicitors chitosan, methyl jasmonate (MeJA) and salicylic acid (SA) as well as the hairy root transformation were tested for silymarin and phenolic compound accumulation in cultures of Milk thistle. For callus induction, leaf explants were cultured on MS medium supplemented with 5 mg/l NAA + 2 mg/l Kin + 0.1 mg/l GA3. Chitosan, SA and MeJA were added separately in three concentrations 200, 400 and 800 mg/l; 10, 20 and 40 mg/l; 20, 40 and 80 mg/l, respectively, to hormone free B5 medium. Alternatively, cotyledons of 12 day old seedlings were transformed with A4 strain. Overall, increasing the concentrations of the three elicitors dramatically increased the total silymarin content. Remarkably, the elicitors mainly enhanced the accumulation of silybine A&B that were not detected in un-treated callus culture (control). In addition, the hairy root culture triggered the accumulation of silybine A&B, and silydianin, which was not detected in the non-transgenic roots. The hairy root culture was superior in production of the phenolic compounds in comparison to the control and elicitor treatments. The hairy root cultures showed also higher antioxidant capacities than non-transformed cultures and/or chemically elicited-callus cultures. Thus hairy root provide instrumental in enhancing the production of economically valuable metabolite.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299848PMC
http://dx.doi.org/10.1016/j.jgeb.2016.10.003DOI Listing

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