() microspore-derived embryogenesis has become a model system to study basic aspects of plant development. Recognized transcription factors governing embryogenesis include: (), a member of the plant-specific B3-domain family, and (), a member of the HAP3 subunit of the CCAAT binding factor family. The effects of altered expression of both genes were investigated during microspore-derived embryogenesis in established lines over-expressing or down-regulating , as well as in tilling lines where was mutated. While over-expression of decreases the yield of microspore-derived embryos (MDEs) without affecting their ability to regenerate plants, suppression of or reduced both embryo number and regeneration frequency. Embryos produced by these lines showed structural abnormalities accompanied by alterations in the expression of several embryogenesis-marker genes. Oil accumulation was also altered in the transgenic MDEs. Total oil content was increased in MDEs over-expressing and decreased in those suppressing or . Mutation of also resulted in a small but significant increase in linoleic (C18:2) acid. Together this study demonstrates the crucial role of and during embryogenesis.
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| http://dx.doi.org/10.1016/j.jgeb.2016.05.002 | DOI Listing |
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