[Effects of Chaihu Shugan Powder on the Cytoactivity of Rat Interstitial Cells of Cajal and Intracellu- lar Ca²+ Concentration].

Objective To observe effects of Chaihu Shugan Powder (CSP) on the cytoactivity and intracellular Ca²⁺ concentration of rat interstitial cells of Cajal (ICCs). Methods CSP containing serum was prepared according to common methods of seropharmacology, which was then divided into three CSP groups (5%,10%, 20% CSP containing serum) , 10% Domperidone containing serum group, and the blank control group. ICCs in gastric antrum were rapidly isolated and extracted to perform routine culture. ICCs were identified by immunofluorescence staining. The logarithmic growth curve of ICCs was determined by MTT method. After 3 days culture of ICCs at the logarithmic phase, the cytoactivity of ICCs was detected by CCK-8 method. Intracellular Ca²⁺ changes of ICCs were tested by Fluo-3 fluorescence with laser scanning confocal microscope. Effects of drug containing serums in each group on the growth of ICCs in gastric an- trum were compared. Results After successful isolation, culture, and identification of ICCs, the cytoac- tivity of ICCs and intracellular Ca²⁺ fluorescence intensity were significantly enhanced in the Domperidone group and the CSP groups, as compared with the blank group at the same time point (P <0. 05). Compared with the Domperidone group at the same time point, the cytoactivity of ICCs and intracellular Ca²⁺ fluorescence intensity were significantly enhanced in 10% and 20% CSP groups (P <0. 05). Compared with 5% CSP group at the same time point, the cytoactivity of ICCs (48 and 72 h respectively) and intracellular Ca²⁺ fluorescence intensity were significantly enhanced in 10% and 20% CSP groups (P <0. 05). Conclu- sion CSP could promote cytoactivity and growth of ICCs by enhancing intracellular Ca²⁺ concentration.