Light sheet illumination is the basis in developing light sheet microscopy (LSM), a technique with significant advantages compared with other classical techniques. Most proposed optical systems to generate light sheets for LSM use many optical elements, which require extensive adjustments and are costly; moreover, they generate a nonuniform or semiuniform light sheet or have a short depth of field (DOF). A simple scheme using a pair of double slits and a cylindrical lens for generating a quasi-nondiffracting 2D light sheet was reported in Opt. Lett.40, 5121 (2015)OPLEDP0146-959210.1364/OL.40.005121. In the present investigation, we elaborate on the optimization of the mask used. As the separation between the two slits increases, the light sheet becomes thinner and the DOF smaller and vice versa. The slits' width does not affect the light sheet thickness, but it does affect the intensity of the side lobes. For convergence angles of the inner slits from 0.75° to 8°, an optimum ratio of the slits' separation/width of 2.182 is recommended. The obtained light sheet is quasi-diffraction-free, namely, while its DOF is comparable with that of a Gaussian beam, its diffraction broadening is substantially smaller. We also add to the previously developed configuration a Powell lens in order to expand the beam in the spanwise direction while keeping nearly constant intensity in this dimension. We perform scalar diffraction theory calculations and conduct measurements showing the nearly constant intensity in the significantly broadened span of the light sheet. Potential applications for the augmented width include imaging of certain large embryos, laser micromachining, and microparticle image velocimetry.
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http://dx.doi.org/10.1364/JOSAA.36.000124 | DOI Listing |
iScience
January 2025
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences (CAS), Xi'an, Shaanxi 710119, China.
Crack pattern-based metal grid film is an ideal candidate material for transparent electromagnetic interference shielding optical windows. However, achieving crack patterns with narrow grid spacing, small wire width, and high connectivity remains challenging. Herein, an aqueous acrylic colloidal dispersion was developed as a crack precursor for preparing crack patterns.
View Article and Find Full Text PDFBMC Med Ethics
January 2025
The Kirby Institute, UNSW Sydney, Sydney, Australia.
Background: The World Professional Association for Transgender Health guidelines Standards of Care 8 draw on ethical arguments based on individual autonomy, to argue that healthcare and other professionals should be advocates for trans people. Such guidelines presume the presence of medical services for trans people and a degree of consensus on medical ethics. Very little is known, however, about the ethical challenges associated with both providing and accessing trans healthcare, including gender affirmation, in the Global South.
View Article and Find Full Text PDFAdv Mater
January 2025
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, P. R. China.
MXenes, have been considered as a new generation anode material in lithium-ion batteries for lower lithium-ion diffusion barriers and superior conductivity. Unfortunately, their structures are prone to aggregation and stacking, hindering further shuttle of lithium ions and electrons, resulting in lower discharge capacity. Therefore, the introduction of interlayer spacers for the preparation of MXene-based hybrids has attracted much attention.
View Article and Find Full Text PDFScience
January 2025
Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
Elucidating the interaction between membrane proteins and antibodies requires whole-cell imaging at high spatiotemporal resolution. Lattice light-sheet (LLS) microscopy offers fast volumetric imaging but suffers from limited spatial resolution. DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) achieves molecular resolution but is restricted to two-dimensional imaging owing to long acquisition times.
View Article and Find Full Text PDFAnalyst
January 2025
Department of Engineering Design, Indian Institute of Technology Madras, India.
High throughput intracellular delivery of biological macromolecules is crucial for cell engineering, gene expression, therapeutics, diagnostics, and clinical studies; however, most existing techniques are either contact-based or have throughput limitations. Herein, we report a light-activated, contactless, high throughput photoporation method for highly efficient and viable cell transfection of more than a million cells within a minute. We fabricated reduced graphene oxide (rGO) nanoflakes that was mixed with a polydimethylsiloxane (PDMS) nanocomposite thin sheet with an area of 3 cm and a thickness of ∼600 μm.
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