Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks.

J Biosci Bioeng

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation (STIN), Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.

Published: June 2019

AI Article Synopsis

  • - The study explored how the yeast Lipomyces starkeyi can produce lipids using inexpensive sap from felled oil palm trunks as a carbon source, which has high sugar content (98 g/L glucose and 32 g/L fructose).
  • - Various fermentation conditions were tested using regular and enriched sap, achieving about 30 g/L of cell biomass and 60% lipid production under all conditions, even in acidic media (pH ≈ 3).
  • - The fatty acid profile of the lipids produced by L. starkeyi closely resembles that of palm oil, making it a potential sustainable feedstock for biodiesel, with oleic acid as the main fatty acid followed by palmitic, stearic, and

Article Abstract

The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.

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Source
http://dx.doi.org/10.1016/j.jbiosc.2018.12.002DOI Listing

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