Purpose: In aging skin and some skin disorders, components of skin extracellular matrix (ECM) are disturbed and therefore research to find skin drugs is important. Evaluation of anethole impact on collagen, GAGs and MMP-2 in human skin fibroblasts was the aim of this study.
Materials And Methods: For collagen assay the Sircol dye, 5-[H]proline and real time-PCR were used. MMP-2 activity was detected by zymography. GAG concentration was determined using 1,9-dimethylmethylene blue (DMMB). Cell viability was assayed with MTT.
Results: In cells treated with 1 and 10 μM anethole, a significant increase in collagen synthesis was demonstrated. In contrast, collagen synthesis was significantly decreased in cells exposed to 100 μM anethole. Similar alterations were found in collagen type I expression. The concentration of collagen secreted into the medium was higher only in cells exposed to 1 μM anethole, while it was lower under the influence of higher compound concentrations. It may be due to the lack of pro-MMP-2 activation at 1 μM and a significant increase in the level of MMP-2 at 10 and 100 μM anethole. GAG concentration was reduced under the influence of 100 μM anethole, whereas anethole at lower concentrations revealed the ability to prevent HO-induced GAG increase. No significant cytotoxicity of anethole to fibroblasts was noted.
Conclusions: Our findings demonstrate the concentration-dependent action of anethole on the crucial components of ECM in cultured skin fibroblasts, which may be somewhat beneficial and may possibly be developed towards a therapeutic use in some skin disorders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.advms.2018.08.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multivalent Neuroprotective Activity of (Cardamom) and (Fennel) in HO-Induced Oxidative Stress in SH-SY5Y Cells and Acellular Assays.
Pharmaceuticals (Basel)
December 2024
Department of Bionano Technology, Gachon Bionano Research Institute, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 461-701, Gyeonggi-do, Republic of Korea.
Background: (Cardamom) and (Fennel) are well-known spices and are also used as natural mouth fresheners. This study was performed to evaluate their neuroprotective ability based on certain acellular and cellular assays.
Methods: Hexane and ethyl acetate extracts were prepared using cardamom and fennel seeds.
Effects of combinations of the essential oils trans-anethole, thymol and carvacrol against larvae of the screwworm fly Cochliomyia hominivorax in vitro.
Vet Parasitol
January 2025
Laboratório de Quimioterapia Experimental em Parasitologia Veterinária (LQEPV), Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil; Departamento de Parasitologia Animal, Instituto de Medicina Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
This study investigated the combined effect of trans-anethole, carvacrol and thymol on third-instar larvae of C. hominivorax. For this experiment, third-stage larvae of C.
View Article and Find Full Text PDFA comprehensive review of the neurological effects of anethole.
IBRO Neurosci Rep
June 2025
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Since ancient times many countries have employed medicinal plants as part of traditional medicine. Anethole is a substance found in various plants and has two isomers, cis-anethole (CA) and trans-anethole (TA). Currently, the food industry extensively use anethole as an aromatic and flavoring component.
View Article and Find Full Text PDFSynchronous Interference of Dual Metabolic Pathways Mediated by HS Gas/GOx for Augmenting Tumor Microwave Thermal Therapy.
ACS Appl Mater Interfaces
January 2025
Key Laboratory of Cryogenics Science and Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Sublethal tumor cells have an urgent need for energy, making it common for them to switch metabolic phenotypes between glycolysis and oxidative phosphorylation (OXPHOS) for compensatory energy supply; thus, the synchronous interference of dual metabolic pathways for limiting energy level is essential in inhibiting sublethal tumor growth. Herein, a multifunctional nanoplatform of Co-MOF-loaded anethole trithione (ADT) and myristyl alcohol (MA), modified with GOx and hyaluronic acid (HA) was developed, namely, CAMGH. It could synchronously interfere with dual metabolic pathways including glycolysis and OXPHOS to restrict the adenosine triphosphate (ATP) supply, achieving the inhibition to sublethal tumors after microwave (MW) thermal therapy.
View Article and Find Full Text PDFEffects of Far-Red Light and Ultraviolet Light-A on Growth, Photosynthesis, Transcriptome, and Metabolome of Mint ( Briq.).
Plants (Basel)
December 2024
College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
To investigate the effects of different light qualities on the growth, photosynthesis, transcriptome, and metabolome of mint, three treatments were designed: (1) 7R3B (70% red light and 30% blue light, CK); (2) 7R3B+ far-red light (FR); (3) 7R3B+ ultraviolet light A (UVA). The results showed that supplemental FR significantly promoted the growth and photosynthesis of mint, as evidenced by the increase in plant height, plant width, biomass, effective quantum yield of PSII photochemistry (F'/F'), maximal quantum yield of PSII (F/F), and performance index (PI). UVA and CK exhibited minimal differences.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!