AI Article Synopsis
- - The study aimed to determine how different concentrations and exposure times of IL-12 impact the gene expression of TNF-α and IFN-γ in Normal Human Dermal Fibroblast (NHDF) cells.
- - Researchers exposed NHDF cells to varying levels of IL-12 and assessed the resulting gene expressions using RTqPCR, finding that changes in one gene's expression often led to the opposite change in the other gene.
- - The findings suggest that monitoring TNF-α and IFN-γ expression could provide insights into the inflammatory response and help evaluate anti-cytokine therapies in treating inflammation.
Article Abstract
Background: The aim of the study was to evaluate the changes in the expression of genes - TNF-α, IFN-γ, depending on the time and concentration of IL-12 used to expose the Normal Human Dermal Fibroblast (NHDF) cells.
Methods: The material for the study included NHDF exposed to IL-12 in various IL-12 concentrations (1/10/100 ng/ml) and exposure time 0.5h, 1h, 2h, 4h, 8h, 24h, compared to the control. Changes in gene expression were evaluated with the use of the RTqPCR method. The statistical analysis was performed with the use of Statistica 12.5 PL The role of genes of the JAK-STAT signaling pathway in the induction of the inflammatory process was determined with the use of the PANTHER overrepresentation test.
Results: Regardless of the time of NHDF exposure and the IL-12 concentrations used, we observed changes in the expressions of TNF-α and IFN-γ. Increased expression of one transcript involves the decreased expression of the other. We assessed that genes of the JAK-STAT signaling pathway are engaged in 6 biological processes, 8 molecular functions, 6 signaling pathways.
Conclusion: The conducted studies indicate that conclusions about the intensity of the inflammatory process, and the efficacy of the anti-cytokine therapeutic strategy, may be made on the basis of the TNF-α, IFN-γ expression.
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| http://dx.doi.org/10.2174/1389201020666190111163312 | DOI Listing |
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