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, an intestinal protozoan pathogen, is one of the leading causes of diarrhea in healthy adults and death in children. Detection of oocysts has become a high priority to prevent potential outbreaks. In this paper, a label-free interdigitated-based capacitive biosensor has been introduced for the detection of oocysts in water samples. Specific anti- monoclonal antibodies (IgG3) were covalently immobilized onto interdigitated gold electrodes as the capture probes, and bovine serum albumin was used to avoid non-specific adsorption. The immobilization of the antibodies was confirmed by measuring the change in the contact angle. The detection was achieved by measuring the relative change in the capacitive/dielectric properties due to the formation of -antibody complex. The biosensor has been tested for different concentrations of . The results show that the biosensor developed can accurately distinguish different numbers of captured cells and densities on the surface of the biosensor. The number of oocysts captured on the electrode surface was confirmed using a fluorescein isothiocyanate (FITC) immunofluorescence assay. The response from the developed biosensor has been mainly dependent on the concentration of under optimized conditions. The biosensor showed a linear detection range between 15 and 153 cells/mm² and a detection limit of 40 cells/mm². The label-free capacitive biosensor developed has a great potential for detecting in environmental water samples. Furthermore, under optimized conditions, this label-free biosensor can be extended for detection of other biomarkers for biomedical and environmental analyses.
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http://dx.doi.org/10.3390/s19020258 | DOI Listing |
Sci Adv
March 2025
Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, P. R. China.
The real-time monitoring of health conditions of humans is a long-lasting topic, but there are two major challenges. First, many biomedical applications accept only implanted sensors. Second, tissue-like soft sensors often suffer from viscoelasticity-induced signal drift, causing inaccurate measurements.
View Article and Find Full Text PDFHeliyon
February 2025
Technical University of Sofia, Dept. of Electronics, 8 Kliment Ohridski Blvd, 1756, Sofia, Bulgaria.
In this paper, it is integrated a piezoelectric energy harvester and a supercapacitor storage device on a flexible substrate with a connection through an innovative alternative current (AC) to direct current (DC) boosting power management system for wearable biosensors' power supply. Flexible substrates can conform to irregular surfaces or shapes, enabling energy harvesting and storage devices to be integrated into a variety of form factors, including curved or bendable surfaces. Having an integrated energy harvester and storage system ensures a reliable and portable power source, providing power autonomy.
View Article and Find Full Text PDFAnal Methods
March 2025
College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, China.
We present a DNA sensor array for simultaneous detection of four targeted miRNAs in serum for early-stage lung cancer screening. Owing to effective microfluidic enrichment coupled with ultrasensitive solid-liquid capacitive sensing, the detection limit is as low as 12.84-24.
View Article and Find Full Text PDFBiosens Bioelectron
June 2025
Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 03722, Seoul, South Korea. Electronic address:
A capacitive-localized plasmon surface resonance (LSPR) dual-mode biosensor was developed using gold (Au) nanoislands modified on an Au interdigitated electrode (IDE). Au nanoislands were deposited through repeated thermal dewetting to increase their packing density and enhance sensor sensitivity. The response of the capacitive sensor to antibody-antigen interactions was optimized at 0.
View Article and Find Full Text PDFACS Sens
February 2025
Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Airborne transmission via aerosols is a dominant route for the transmission of respiratory pathogens, including avian H5N1 influenza A virus and bacteria. Rapid and direct detection of respiratory pathogen aerosols has been a long-standing technical challenge. Herein, we develop a novel label-free capacitive biosensor using an interlocked Prussian blue (PB)/graphene oxide (GO) network on a screen-printed carbon electrode (SPCE) for direct detection of avian H5N1 and .
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