Identification of Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method.

Fascioliasis-a disease caused by spp. (Platyhelminthes: Trematoda: Digenea)-is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of spp. isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Adult spp. were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam. Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 7 samples from infected bovine and 89 samples from infected buffalo. 96/96 samples were identified as species by ITS1 of rDNA. In this study, a PCR-RFLP method was used to distinguish between and in ITS1 of rDNA (680 bp) with I restriction enzyme. RFLP pattern with I produced a consistent pattern of 360, 100, and 60 bp fragments in , whereas worms had a profile of 360, 170, and 60 bp in size, respectively. The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of type, whereas three isolates presented an intermediate . In the present study, and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas was not detected.