AI Article Synopsis
- This study outlines a method for creating signaling aptamers using SELEX and next-generation sequencing to screen various candidates.
- The researchers identified promising DNA signaling aptamers specifically targeting VEGFR-1, an important receptor in signaling pathways.
- This innovative approach simplifies the discovery process for signaling aptamers by reducing the need for complex modifications and optimizations.
Article Abstract
Here, we demonstrated a strategy for developing signaling aptamers, based on screening of signaling aptamers from multiple aptamer candidates obtained by SELEX with next generation sequencing. Among aptamer candidates labelled by 6-carboxyfluorescein and quencher at both end termini, there is the possibility of discovering a potent signaling aptamer. In this study, we discovered DNA signaling aptamers against VEGFR-1. This strategy has the potential for signaling aptamer discovery without the extremely laborious task of optimization of oligodeoxynucleotide modifications.
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| http://dx.doi.org/10.2116/analsci.18SDN05 | DOI Listing |
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