AI Article Synopsis
- Mimotopes, which can replace harmful mycotoxins, are being used to develop new immunoassay methods with a focus on aflatoxin B1 (AFB).
- Researchers created a magnetic beads-based competitive ELISA (MB-dcELISA) that effectively utilizes a specific nanobody (Nb28) and its mimotope (ME17) for detection.
- The new immunoassay demonstrated strong performance with low detection limits and good recovery rates in various food samples, offering a promising strategy for monitoring AFB and similar toxic compounds.
Article Abstract
Mimotopes could replace mycotoxins and their conjugates to develop immunoassay methods. The mimotopes obtained by phage display technology were mainly using monoclonal antibodies or polyclonal antibodies as targets. However, the mimotope of recombinant antibody has not been selected and applied to immunoassay for mycotoxin. The purpose of this study was to prove that an immunoassay for mycotoxin could be developed based on both recombinant antibody and its mimotope. Using aflatoxin B1 (AFB) as a model system, mimotopes of an aflatoxin nanobody Nb28 were screened by phage display. A rapid magnetic beads-based directed competitive ELISA (MB-dcELISA) was developed utilizing Nb28 and its mimotope ME17. The 50% inhibitory concentration and the detection limit of the MB-dcELISA were 0.75 and 0.13 ng/mL, respectively, with a linear range of 0.24-2.21 ng/mL. Further validation study indicated good recovery (84.2-116.2%) with low coefficient of variable (2.2%-15.9%) in spiked corn, rice, peanut, feedstuff, corn germ oil and peanut oil samples. The developed immunoassay based on nanobody and mimotope provides a new strategy for the monitoring of AFB and other toxic small molecular weight compounds.
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| http://dx.doi.org/10.1016/j.talanta.2018.11.013 | DOI Listing |
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